anti-pstat1 rabbit polyclonal antibody (Proteintech)
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Anti Pstat1 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti-pstat1+rabbit+polyclonal+antibody/anti+pstat1+rabbit+polyclonal+antibody/pmc11445893-272-47-52
Average 90 stars, based on 1 article reviews
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1) Product Images from "Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib"
Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
Journal: Gut Microbes
doi: 10.1080/19490976.2024.2410474
Figure Legend Snippet: Efm enhanced the ability of sorafenib to induce ferroptosis in hepatocellular carcinoma. (a) The relative protein expression of SLC7A11, STAT1 and pSTAT1 were assessed by western blotting. (b,c) the binding sites between STAT1 and SLC7A11 predicted by JASPAR database. (d) SLC7A11 mRNA levels in tumor tissues with different treatment were detected by qRT-pcr analysis. (e) Representative immunofluorescence staining of total ROS (green) in tumor tissues with different treatment. (f) The morphology of mitochondrial ridges (marked by red arrows) in tumor tissues with different treatment were evaluated by transmission electron microscopy. Each dot represents a mouse. Error bars represent the SEM. ns p >0.05, * p <0.05, ** p <0.01, *** p <0.001.
Techniques Used: Expressing, Western Blot, Binding Assay, Quantitative RT-PCR, Immunofluorescence, Staining, Transmission Assay, Electron Microscopy
Figure Legend Snippet: Schematic illustration of EPS secretion by efm to induce IFN-γ + CD8 + T cells to promote ferroptosis induced by sorafenib in hepatocellular carcinoma. The EPS secreted by efm in the intestine enters the tumor microenvironment of HCC and prompts IFN-γ + CD8 + T cells to secrete ifn-γ. ifn-γ enters HCC cells to activate the JAK-STAT1 signaling pathway and produce phosphorylated STAT1, which acts as a transcription factor to inhibit the transcription of SLC7A11, thereby increasing intracellular ROS and inducing ferroptosis in HCC cells.
Techniques Used:
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Blocking Assay:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Incubation:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Expressing:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Western Blot:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Binding Assay:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Quantitative RT-PCR:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Immunofluorescence:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Staining:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Transmission Assay:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Electron Microscopy:Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib. Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), |