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anti-pstat1 rabbit polyclonal antibody  (Proteintech)


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    Structured Review

    Proteintech anti-pstat1 rabbit polyclonal antibody
    Efm enhanced the ability of sorafenib to induce ferroptosis in hepatocellular carcinoma. (a) The relative protein expression of SLC7A11, STAT1 and <t>pSTAT1</t> were assessed by western blotting. (b,c) the binding sites between STAT1 and SLC7A11 predicted by JASPAR database. (d) SLC7A11 mRNA levels in tumor tissues with different treatment were detected by qRT-pcr analysis. (e) Representative immunofluorescence staining of total ROS (green) in tumor tissues with different treatment. (f) The morphology of mitochondrial ridges (marked by red arrows) in tumor tissues with different treatment were evaluated by transmission electron microscopy. Each dot represents a mouse. Error bars represent the SEM. ns p >0.05, * p <0.05, ** p <0.01, *** p <0.001.
    Anti Pstat1 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti-pstat1+rabbit+polyclonal+antibody/anti+pstat1+rabbit+polyclonal+antibody/pmc11445893-272-47-52
    Average 90 stars, based on 1 article reviews
    anti-pstat1 rabbit polyclonal antibody - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib"

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib

    Journal: Gut Microbes

    doi: 10.1080/19490976.2024.2410474

    Efm enhanced the ability of sorafenib to induce ferroptosis in hepatocellular carcinoma. (a) The relative protein expression of SLC7A11, STAT1 and pSTAT1 were assessed by western blotting. (b,c) the binding sites between STAT1 and SLC7A11 predicted by JASPAR database. (d) SLC7A11 mRNA levels in tumor tissues with different treatment were detected by qRT-pcr analysis. (e) Representative immunofluorescence staining of total ROS (green) in tumor tissues with different treatment. (f) The morphology of mitochondrial ridges (marked by red arrows) in tumor tissues with different treatment were evaluated by transmission electron microscopy. Each dot represents a mouse. Error bars represent the SEM. ns p >0.05, * p <0.05, ** p <0.01, *** p <0.001.
    Figure Legend Snippet: Efm enhanced the ability of sorafenib to induce ferroptosis in hepatocellular carcinoma. (a) The relative protein expression of SLC7A11, STAT1 and pSTAT1 were assessed by western blotting. (b,c) the binding sites between STAT1 and SLC7A11 predicted by JASPAR database. (d) SLC7A11 mRNA levels in tumor tissues with different treatment were detected by qRT-pcr analysis. (e) Representative immunofluorescence staining of total ROS (green) in tumor tissues with different treatment. (f) The morphology of mitochondrial ridges (marked by red arrows) in tumor tissues with different treatment were evaluated by transmission electron microscopy. Each dot represents a mouse. Error bars represent the SEM. ns p >0.05, * p <0.05, ** p <0.01, *** p <0.001.

    Techniques Used: Expressing, Western Blot, Binding Assay, Quantitative RT-PCR, Immunofluorescence, Staining, Transmission Assay, Electron Microscopy

    Schematic illustration of EPS secretion by efm to induce IFN-γ + CD8 + T cells to promote ferroptosis induced by sorafenib in hepatocellular carcinoma. The EPS secreted by efm in the intestine enters the tumor microenvironment of HCC and prompts IFN-γ + CD8 + T cells to secrete ifn-γ. ifn-γ enters HCC cells to activate the JAK-STAT1 signaling pathway and produce phosphorylated STAT1, which acts as a transcription factor to inhibit the transcription of SLC7A11, thereby increasing intracellular ROS and inducing ferroptosis in HCC cells.
    Figure Legend Snippet: Schematic illustration of EPS secretion by efm to induce IFN-γ + CD8 + T cells to promote ferroptosis induced by sorafenib in hepatocellular carcinoma. The EPS secreted by efm in the intestine enters the tumor microenvironment of HCC and prompts IFN-γ + CD8 + T cells to secrete ifn-γ. ifn-γ enters HCC cells to activate the JAK-STAT1 signaling pathway and produce phosphorylated STAT1, which acts as a transcription factor to inhibit the transcription of SLC7A11, thereby increasing intracellular ROS and inducing ferroptosis in HCC cells.

    Techniques Used:

    Related Articles

    Blocking Assay:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Incubation:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Expressing:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Western Blot:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Binding Assay:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Quantitative RT-PCR:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Immunofluorescence:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Staining:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Transmission Assay:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Electron Microscopy:

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib.
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib
    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).



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    Image Search Results


    Efm enhanced the ability of sorafenib to induce ferroptosis in hepatocellular carcinoma. (a) The relative protein expression of SLC7A11, STAT1 and pSTAT1 were assessed by western blotting. (b,c) the binding sites between STAT1 and SLC7A11 predicted by JASPAR database. (d) SLC7A11 mRNA levels in tumor tissues with different treatment were detected by qRT-pcr analysis. (e) Representative immunofluorescence staining of total ROS (green) in tumor tissues with different treatment. (f) The morphology of mitochondrial ridges (marked by red arrows) in tumor tissues with different treatment were evaluated by transmission electron microscopy. Each dot represents a mouse. Error bars represent the SEM. ns p >0.05, * p <0.05, ** p <0.01, *** p <0.001.

    Journal: Gut Microbes

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib

    doi: 10.1080/19490976.2024.2410474

    Figure Lengend Snippet: Efm enhanced the ability of sorafenib to induce ferroptosis in hepatocellular carcinoma. (a) The relative protein expression of SLC7A11, STAT1 and pSTAT1 were assessed by western blotting. (b,c) the binding sites between STAT1 and SLC7A11 predicted by JASPAR database. (d) SLC7A11 mRNA levels in tumor tissues with different treatment were detected by qRT-pcr analysis. (e) Representative immunofluorescence staining of total ROS (green) in tumor tissues with different treatment. (f) The morphology of mitochondrial ridges (marked by red arrows) in tumor tissues with different treatment were evaluated by transmission electron microscopy. Each dot represents a mouse. Error bars represent the SEM. ns p >0.05, * p <0.05, ** p <0.01, *** p <0.001.

    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Techniques: Expressing, Western Blot, Binding Assay, Quantitative RT-PCR, Immunofluorescence, Staining, Transmission Assay, Electron Microscopy

    Schematic illustration of EPS secretion by efm to induce IFN-γ + CD8 + T cells to promote ferroptosis induced by sorafenib in hepatocellular carcinoma. The EPS secreted by efm in the intestine enters the tumor microenvironment of HCC and prompts IFN-γ + CD8 + T cells to secrete ifn-γ. ifn-γ enters HCC cells to activate the JAK-STAT1 signaling pathway and produce phosphorylated STAT1, which acts as a transcription factor to inhibit the transcription of SLC7A11, thereby increasing intracellular ROS and inducing ferroptosis in HCC cells.

    Journal: Gut Microbes

    Article Title: Synergistic activity of Enterococcus Faeciu m-induced ferroptosis via expansion of IFN-γ + CD8 + T cell population in advanced hepatocellular carcinoma treated with sorafenib

    doi: 10.1080/19490976.2024.2410474

    Figure Lengend Snippet: Schematic illustration of EPS secretion by efm to induce IFN-γ + CD8 + T cells to promote ferroptosis induced by sorafenib in hepatocellular carcinoma. The EPS secreted by efm in the intestine enters the tumor microenvironment of HCC and prompts IFN-γ + CD8 + T cells to secrete ifn-γ. ifn-γ enters HCC cells to activate the JAK-STAT1 signaling pathway and produce phosphorylated STAT1, which acts as a transcription factor to inhibit the transcription of SLC7A11, thereby increasing intracellular ROS and inducing ferroptosis in HCC cells.

    Article Snippet: After blocking for 15 min with a quickblock buffer (Beyotime, P0235; Shanghai, China), the primary antibody was used upon an overnight incubation at 4°C, including Anti -Interferon γ Rabbit pAb (1:500, Servicebio, GB11107–1), Anti-xCT Rabbit monoclonal antibody (1:1000, Abcam, ab175186), anti-STAT1 Rabbit polyclonal antibody (1:2000, Proteintech 10,144–2-AP), anti-pSTAT1 Rabbit polyclonal antibody (1:1000, Proteintech 28,977–1-AP)and Anti-GAPDH Rabbit monoclonal antibody (1:5000, Abcam, ab181602).

    Techniques: